Bioneer’s AccuPower PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq†Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include HotStart PCR, or High Fidelity PCR, please see our AccuPower HotStart PCR product pages. AccuPower PreMix PCR master mix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer. Learn More
AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.
Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.
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AccuPower® ProFi Taq PCR PreMix is a convenient lyophilized PCR master mix containing ProFi Taq DNA polymerase, reaction buffer, dNTPs, tracking dye, and a patented stabilizer. ProFi Taq DNA polymerase in the premix is a unique recombinant Taq DNA polymerase that offers enhanced amplification efficiency and higher fidelity for PCR. AccuPower® ProFi Taq PCR PreMix is applicable to any template DNA, and especially effective in amplifying large genomic DNA fragments around 20 kb. AccuPower® ProFi Taq PCR PreMix provides accurate long-range amplification of standard and amplification of low-copy target, and is highly suitable for all PCR applications. Learn More
AccuPower® Pfu PCR PreMix is a lyophilized mixture of Pfu DNA polymerase, dNTPs and reaction buffer in a convenient premix format. Simply add template, primers and water and mix – AccuPower® offers easy set-up for every PCR application. Bioneer's patented stabilizer maintains the activity of the PreMix for over a month when stored at room temperature (25°C) and for over 2 years in the freezer (-20°C). Learn More
AccuPower® Gold Multiplex PCR PreMix can amplify up to 20 target genes in a single tube. AccuPower® Gold Multiplex PCR PreMix contains Bioneer’s unique enzyme-mediated HotStart technology with Pyrophosphatase (PPase) and Pyrophosphate (PPi) for efficient suppression of non-specific products and enhanced amplification specificity.
AccuPower® Gold Multiplex PCR PreMix can be used for a variety of applications including genotyping assays or molecular diagnostics, and can also be used for cDNA-based semi-quantitative assays. Learn More
The AccuPower RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.
For cDNA amplification with Cyclic RT, please see our AccuPower CycleScript RT PreMix. Learn More
AccuPower® GreenStar™ RT-qPCR PreMix & Master Mix detects desired target gene precisely from various samples. This real-time PCR reagent using the dsDNA binding dye-based product measures gene amplification at the same time through fluorescence evaluation at each cycle. It has a feature of selective amplification of target cDNA from tiny amount of template RNA, and is useful for viral RNA detection or for quantitative gene expression. Learn More
The AccuPower® Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes.
The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer.
AccuPower® Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer. Learn More
AccuPower RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low copy RNA or mRNA and subsequent RT-PCR . Learn More
