Bioneer’s AccuPrep 96 Genomic DNA Extraction Kit allows for the simultaneous genomic dna purification from 96 samples of cultured cells, whole blood, buffy coat, lymphocytes, plasma, serum, and other biological fluids. Yield is sample dependent, but is typically 6 μg per 200 µl healthy whole human blood.
The AccuPrep 96 Genomic DNA Extraction Kit is based on the principle of DNA binding to a silica membrane in the presence of Chaotropic salts. After lysis of the cells in a Proteinase K buffer, AccuPrep binding buffer is added and the sample is transferred to the 96 well binding plate. Purification is accomplished with subsequent washes with the BioVac 96 Vacuum Manifold. High-purity gDNA is then obtained by eluting the gDNA in AccuPrep EL Buffer, which is then ready for immediate use in downstream applications. Learn More
Bioneer’s AccuPrep 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer oligonucleotides and primer dimers found in PCR reactions are easily removed. Removal of mineral oil is not necessary.
The principle of the AccuPrep 96 PCR Purification Kit is based on the adsorption of DNA in glass filter. Purification is accomplished with the BioVac 96 Vacuum Manifold. Chaotropic salt enhances the binding of DNA onto a glass filter that is fixed in the AccuPrep 96 Binding plate. Adsorption of DNA is very selective so that other proteins and salts are not adsorbed and pass through the glass filter. Washing eliminates salts and other proteins that may bind non-specifically. High-purity DNA fragments are eluted with provided AccuPrep EL Buffer (10 mM Tris-Cl, pH 8.5) or distilled water. Purified DNA fragment can be immediately used for subcloning, sequencing and other molecular biological applications. Learn More
